Functional circuits of LYL1 controlled by supraphysiological androgen in prostate cancer cells to regulate cell senescence.

ABSTRACT:

Background

Prostate cancer (PCa) is a public health problem mostly reported in developed countries. The androgen receptor (AR) regulates the development and physiological function of normal prostate as well as the proliferation of cancerous prostate tissue. Treatment with supraphysiological androgen levels (SAL) is used in bipolar androgen therapy and inhibits PCa growth, suggesting SAL induces a tumor suppressive program. It was shown that SAL induces cellular senescence, in PCa cell lines, human tumor samples and in xenografted mouse tumor model.

Methods

Transcriptome and ChIP-seq analysis, PCa spheroids, knockdown (KD), co-immunoprecipitation, qRT-PCR, immune detection, in situ histochemistry.

Results

Here we show that LYL1 is upregulated by the clock gene BHLHE40

SUBMITTER: Heidari Horestani M 

PROVIDER: S-EPMC11636232 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature