TPM4 overexpression drives colon epithelial cell tumorigenesis by suppressing differentiation and promoting proliferation.

ABSTRACT:

Objective

The high morbidity and mortality associated with colorectal cancer (CRC) and the recent increases in early-onset CRC obviate the need for novel methods to detect and treat this disease, particularly at early stages. We hypothesize that aberrant expression of genes involved in the crypt-luminal migration of colon epithelial cells, a process necessary for their growth arrest and maturation, may disrupt differentiation and transition cells from a normal to tumorigenic state.

Methods

We searched for contractility- and motility-related genes that are dysregulated in human CRC relative to normal colon. RNA expression of one such gene, tropomyosin 4 (TPM4), was measured by qRT-PCR and RNA-seq in human colorectal tissues at various stages of tumorigenesis: CRC, adenoma, an

SUBMITTER: Macwan RS 

PROVIDER: S-EPMC11636349 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature