Impact of various cryo-preservation steps on sperm rheotaxis and sperm kinematics in bull.

ABSTRACT: Semen cryopreservation is an important tool that has massively contributed to the progression of animal reproduction, especially in cattle. Nonetheless, a large part of the sperm population suffers from cryostress and loses fertility during the process. Although bovine semen cryopreservation is more advanced than any other species, there are still some missing links in the technology knowledge. The aim of the current study was to detect the effect of cryopreservation steps on sperm rheotaxis. Semen samples were collected from sex bulls and analyzed inside a microfluidic platform with CASA after each step of cryopreservation, including control, dilution with yolk citrate, cryoprotectant addition, and cooling or freezing. The results showed that positive rheotaxis % (PR) was not affected dur

SUBMITTER: Mofadel HA 

PROVIDER: S-EPMC11636841 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature