O304 alleviates abdominal aortic aneurysm formation via AMPK/mTOR/MMP pathway activation.

ABSTRACT:

Background

Abdominal aortic aneurysm (AAA) rupture is a significant cause of mortality in the elderly population. Despite experimental models identifying promising pharmacological therapies, there is still a lack of pharmacological interventions for AAA prior to surgery. This study aims to evaluate the regulatory role of the novel adenosine monophosphate-activated protein kinase (AMPK) agonist O304 in AAA formation and explore its underlying molecular mechanisms.

Methods

We evaluated the expression of AMPK signaling pathway components and contractile vascular smooth muscle cell (VSMC)-related genes in AAA samples from mice using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We evaluate the TGF-β expression by western blotting and RT-qPCR and TGF-β c

SUBMITTER: Sun D 

PROVIDER: S-EPMC11637863 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature