Small-Angle X-ray Scattering (SAXS) Combined with SAXS-Driven Molecular Dynamics for Structural Analysis of Multistranded RNA Assemblies.

ABSTRACT: Nucleic acids (RNA and DNA) play crucial roles in all living organisms and find wide utility in clinical settings. The convergence of rationally designed nucleic acid multistranded assemblies with embedded therapeutic properties has led to the development of a platform based on nucleic acid nanoparticles (NANPs). NANPs incorporate various functional moieties to deliver their combinations to diseased cells in a highly controlled manner. Given that the structure and composition of NANPs can also influence their immunorecognition and biological activities, thorough verification of all designs is essential. We introduce an experimental pipeline for small-angle X-ray scattering (SAXS) to gather structural details about the solution-state NANPs assembled from up to 12 RNA strands. To the best of

SUBMITTER: Rolband LA 

PROVIDER: S-EPMC11637918 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature