Characterisation of the genetic diversity of citrus viroid VII using amplicon sequencing.
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ABSTRACT: Viroids occur in plants as swarms of sequence variants clustered around a dominant variant, leading to adoption of the term 'quasispecies' to describe the viroid population in an individual host. The composition of the quasispecies can potentially change according to the age of the infection, the position of the leaf or branch in the canopy, and the host species. The primary aim of this study was to investigate the quasispecies concept for citrus viroid VII (CVd-VII), a recently discovered member of the family Pospiviroidae. Three experiments were conducted to determine factors affecting viroid variability (i) within different tissues of a lemon plant, (ii) among different plants of the same species (citron), and (iii) among different species and hybrids of citrus. Using two primer sets to
SUBMITTER: Chambers GA
PROVIDER: S-EPMC11638337 | biostudies-literature | 2024 Dec
REPOSITORIES: biostudies-literature
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