Ex vivo-generated human CD1c⁺ regulatory B cells by a chemically defined system suppress immune responses and alleviate graft-versus-host disease.

ABSTRACT: IL-10⁺ regulatory B cells (Bregs) show great promise in treating graft-versus-host disease (GVHD), a life-threatening complication of post-hematopoietic stem cell transplantation. However, obtaining high-quality human IL-10⁺ Bregs in vitro remains a challenge due to the lack of unique specific markers and the triggering of pro-inflammatory cytokine expression. Here, by uncovering the critical signaling pathways in Breg induction by mesenchymal stromal cells (MSCs), we first established an efficient Breg induction system based on MSCs and GSK-3β blockage (CHIR-99021), which had a robust capacity to induce IL-10⁺ Bregs while suppressing tumor necrosis factor α (TNF-α) expression. Furthermore, these Breg populations could be identified and enriched by CD1c

SUBMITTER: Bao Y 

PROVIDER: S-EPMC11638867 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature