Extrinsic electric field modulates neuronal development and increases photoreceptor population in retinal organoids.

ABSTRACT:

Introduction

Considering the significant role played by both intrinsic and extrinsic electric fields in the growth and maturation of the central nervous system, the impact of short exposure to external electric fields on the development and differentiation of retinal organoids was investigated.

Methods

Retinal organoids derived from human embryonic stem cells were used at day 80, a key stage in their differentiation. A single 60-minute exposure to a biphasic electrical field was administered to assess its influence on retinal cell populations and maturation markers. Immunohistochemistry, qPCR, and RNA sequencing were employed to evaluate cell type development and gene expression changes.

Results

Electrical stimulation significantly enhanced neuronal development and in

SUBMITTER: Rajendran Nair DS 

PROVIDER: S-EPMC11639233 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature