Improving Quantitative Accuracy in Nontargeted Lipidomics by Evaluating Adduct Formation.

ABSTRACT: For large-scale lipidomic analyses, accurate and reproducible quantification of endogenous lipids is crucial for comparing results within and across studies. Many lipids present in liquid chromatography-electrospray ionization-mass spectrometry form various adducts with buffer components. The mechanisms and conditions that dictate adduct formation are still poorly understood. In a positive mode, neutral lipids like mono-, di-, and triacylglycerides and cholesteryl esters typically generate [M + NH₄]⁺ adduct ions, although [M + Na]⁺, [M + K]⁺, and other (more complex) species can also be significantly abundant in MS1 precursor ion spectra. Variations in the ratios of these adducts (within and between matrices) can lead to dramatic inaccuracies dur

SUBMITTER: Bishop LM 

PROVIDER: S-EPMC11639661 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature