Characterization of Feline Basophils on the Sysmex XN-1000V and Evaluation of a New WDF Gating Profile.

ABSTRACT: The Sysmex XN-1000V provides a percentage and concentration of basophils from the WNR scattergram, as for human samples, but this method has been shown to be irrelevant in cats. This study aimed to characterize the feline basophil distribution on the WDF channel and to preliminarily evaluate the performance of a new basophil gate on the WDF channel. Cases of feline basophilia were retrospectively retrieved and the scattergram from the WDF and WNR channels were evaluated for any consistent pattern. A new gating setting for the WDF channel was created to include the suspected basophil region. This new gating was applied retrospectively to identified basophilia cases and prospectively to randomly selected feline cases. Manual, WNR, and new WDF methods for basophil identification were compared

SUBMITTER: Martinez-Caro J 

PROVIDER: S-EPMC11639760 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature