Integrating Bacteriophage onto the Magnetic Nanozyme for Effective Enrichment and Colorimetric Detection of <i>Cronobacter sakazakii</i> in Powdered Infant Formula.

ABSTRACT: Cronobacter sakazakii is a dangerous pathogen easily found in powdered infant formula (PIF), causing severe infections and even death in infants. Herein, a bacteriophage-immobilized magnetic nanozyme (Fe₃O₄@EspYZU13) was prepared for C. sakazakii detection. Bacteriophage EspYZU13 isolated and identified by our group exhibits specific lytic capacity. Fe₃O₄@EspYZU13 possesses remarkable enrichment capacity towards C. sakazakii, efficiently enriching different concentrations of C. sakazakii from a mixed bacterial solution. Furthermore, Fe₃O₄@EspYZU13 shows peroxidase-like activity, which can catalyze the 3,3',5,5'-tetramethylbenzidine (TMB) chromogenic reaction in the presence of H₂O₂

SUBMITTER: Xu X 

PROVIDER: S-EPMC11639770 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature