Prolactin Inhibition Promotes Follicle Recruitment by Increasing PIKfyve Expression in Ewes During the Estrus Stage.

ABSTRACT: Prolactin (PRL) plays a key role in the growth and ovulation of animal follicles, but its impact on follicular recruitment in ewes remains uncertain. In this study, a total of sixteen healthy ewes (Hu sheep, aged 2-3 years, with continuous reproduction and housed separately), matched for parity and weight (52.98 ± 0.96 kg), were randomly assigned to two groups: a control group (C) and a treatment group (T, PRL inhibition). Ovaries were collected in vivo after anesthesia during the estrus stage, and tissue morphology was observed using hematoxylin-eosin (HE) staining. By using RNA sequencing on the ovaries of C and T groups and conducting bioinformatics analysis, the essential genes and pathways involved in the regulation of PRL inhibition were pinpointed. Subcellular localization of key ge

SUBMITTER: Yue S 

PROVIDER: S-EPMC11639777 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature