Bovine NMRAL2 Protein Blunts Nitric Oxide Production and Inflammatory Response in <i>Mycobacterium bovis</i> Infected Bovine Lung Epithelial Cells.

ABSTRACT: Tuberculosis (TB), primarily caused by Mycobacterium tuberculosis (M. tb) and Mycobacterium bovis (M. bovis), remains the leading cause of death from a single infectious agent globally. Intracellular survival is crucial for their virulence; yet, the underlying mechanisms are not fully understood. This study aimed to demonstrate the significance of a previously unannotated bovine gene ENSBTAG00000011305 in M. bovis intracellular survival. This gene was termed NMRAL2_Bovine due to its inclusion of the NmrA domain which has a relation to nitric oxide (NO) production. We used CRISPR/Cas9 to knock out NMRAL2_Bovine in bovine lung epithelial cells and observed a significant decrease in M. bovis-induced cell death and the intracellular bacte

SUBMITTER: Peng Y 

PROVIDER: S-EPMC11640032 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature