Development of a Simple IFN-γ Release Whole Blood Assay for the Assessment of <i>Leishmania infantum</i> Specific Cellular Immunity in Dogs.

ABSTRACT: Canine leishmaniosis (CanL) is a zoonotic disease caused by Leishmania infantum, where increased interferon-gamma (IFN-γ) levels are associated with controlling the infection and mild to moderate disease. Therefore, monitoring IFN-γ concentrations is essential for monitoring the immune responses in CanL. This study compared a faster, cost-effective IFN-γ release whole blood assay in tubes (WBA-T) with a standardized version (WBA-S) in 41 dogs at different states of L. infantum infection. WBA-T was performed at 24, 48, and 72 h of incubation with three conditions: blood, blood with L. infantum-soluble antigen (LSA), and blood with mitogen ConA. WBA-S was performed in plates, with blood diluted and incubated for five days using the same conditions. Supernatants (WBA-S) o

SUBMITTER: Molnar AS 

PROVIDER: S-EPMC11640336 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature