Direct Interaction Between CD34⁺ Hematopoietic Stem Cells and Mesenchymal Stem Cells Reciprocally Preserves Stemness.

ABSTRACT:

Background/objectives

A specialized microenvironment in the bone marrow, composed of stromal cells including mesenchymal stem cells (MSCs), supports hematopoietic stem cell (HSC) self-renewal, and differentiation bands play an important role in leukemia development and progression. The reciprocal direct interaction between MSCs and CD34⁺ HSCs under physiological and pathological conditions is yet to be fully characterized.

Methods

Here, we established a direct co-culture model between MSCs and CD34⁺ HSCs or MSCs and acute myeloid leukemia cells (THP-1, Molm-13, and primary cells from patients) to study heterocellular communication.

Results

Following MSCs-CD34⁺ HSCs co-culture, the expression of adhesion markers N-Cadherin and connexin

SUBMITTER: Safi R 

PROVIDER: S-EPMC11640414 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature