Flow Cytometry Analyses of Meningioma Immune Cell Composition Using a Short, Optimized Digestion Protocol.

ABSTRACT:

Background

Current challenges in meningioma treatment, including post-surgical complications and cognitive impairments, highlight the need for new treatment alternatives. Immunological interventions have shown promise. However, there is a knowledge gap in characterizing infiltrating immune cells in meningioma and their interplay. Further studies on immune cells in single-cell suspensions from digested meningioma tissues could identify targetable mechanisms for non-surgical treatment options with fewer side effects. This study aimed to optimize a protocol for faster digestion of meningioma tissues into viable single-cell suspensions and to identify infiltrating immune cell populations.

Methods

We modified a commercial kit intended for whole skin dissociation to digest resecte

SUBMITTER: Dao Nyesiga G 

PROVIDER: S-EPMC11640484 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature