Effects of TRPC1's Lysines on Heteromeric TRPC5-TRPC1 Channel Function.

ABSTRACT:

Background

TRPC5 proteins form plasma membrane cation channels and are expressed in the nervous and cardiovascular systems. TRPC5 activation leads to cell depolarization and increases neuronal excitability, whereas a homologous TRPC1 inhibits TRPC5 function via heteromerization. The mechanism underlying the inhibitory effect of TRPC1 in TRPC5/TRPC1 heteromers remains unknown.

Methods

We used electrophysiological techniques to examine the roles of subunit stoichiometry and positively charged luminal residues of TRPC1 on TRPC5/TRPC1 function. We also performed molecular dynamics simulations.

Results

We found that increasing the relative amount of TRPC1 in TRPC5/TRPC1 heteromers reduced histamine-induced cation influx through the heteromeric channels. Consistently, hista

SUBMITTER: Demaree IS 

PROVIDER: S-EPMC11640535 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature