Mesoscopic axially swept oblique plane microscope for the imaging of freely moving organisms with near-isotropic resolution.

ABSTRACT: Rapid three-dimensional imaging over extended fields of view (FOVs) is crucial to the study of organism-wide systems and biological processes in vivo. Selective-plane illumination microscopy (SPIM) is a powerful method for high spatio-temporal resolution in toto imaging of such biological specimens. However, typical SPIM implementations preclude conventional sample mounting and have anisotropic imaging performance, in particular when designed for large FOVs over 1 mm diameter. Here, we introduce axial sweeping of the illumination into a non-orthogonal dual-objective oblique plane microscope (OPM) design, thereby enabling the observation of freely moving animals over millimeter-sized FOVs, at close to isotropic, sub-cellular resolution. We apply our mesoscopic axially swept OP

SUBMITTER: Davis S 

PROVIDER: S-EPMC11640566 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature