Spatial polarimetric second harmonic generation evaluation of collagen in a hypophosphatasia mouse model.

ABSTRACT: Polarization-resolved second harmonic generation (pSHG) is a label-free method that has been used in a range of tissue types to describe collagen orientation. In this work, we develop pSHG analysis techniques for investigating cranial bone collagen assembly defects occurring in a mouse model of hypophosphatasia (HPP), a metabolic bone disease characterized by a lack of bone mineralization. After observing differences in bone collagen lamellar sheet structures using scanning electron microscopy, we found similar alterations with pSHG between the healthy and HPP mouse collagen lamellar sheet organization. We then developed a spatial polarimetric gray-level co-occurrence matrix (spGLCM) method to explore polarization-mediated textural differences in the bone collagen mesh. We used our spGLCM

SUBMITTER: Zheng T 

PROVIDER: S-EPMC11640570 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature