Microfluidics-based automatic immunofluorescence staining for single-molecule localization microscopy.

ABSTRACT: Full automation of single-molecule localization microscopy (SMLM) is crucial for large-scale and high-throughput cellular imaging. It is well-known that SMLM typically consists of three major steps: immunofluorescence (IF) staining, optical imaging, and image processing. Currently, automation in optical imaging and image processing is almost complete; however, the automation of IF staining has been slow to advance, probably due to its complicated experimental operations. Here we present a low-cost automated method for IF staining, called super-resolution immunofluorescence staining by microfluidics (SRIF-fluidics). This method is suitable for both adherent and suspension cells and supports single-color and multi-color IF staining for SMLM. Our results show that SRIF-fluidics reduces antibo

SUBMITTER: Dong J 

PROVIDER: S-EPMC11640577 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature