Diffractive hyperchromatic objective for chromatic confocal microscopy.

ABSTRACT: The limited focal shift of conventional achromatic objectives constrains the maximum imaging depth of chromatic confocal microscopes. To address this, we designed a hyperchromatic confocal microscope using diffractive optical elements, which was fabricated by single-point diamond turning (SPDT). This design takes advantage of the small Abbe number of diffractive optical elements to introduce a significant longitudinal chromatic shift. The resulting chromatic confocal microscope achieved a maximum imaging depth of 750 µm and a lateral resolution of 0.78 µm across a wavelength range of 600-810 nm. The system's imaging capabilities were demonstrated by capturing detailed images of biological samples, including cucumber seed cavities, pig kidney, and human forearm skin. These results confirmed

SUBMITTER: Chen J 

PROVIDER: S-EPMC11640585 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature