Recognition of mismatched sites in double-stranded DNA by a pair of partially noncomplementary peptide nucleic acids.

ABSTRACT: We have successfully achieved efficient recognition of mismatched sites in double-stranded DNA through the formation of an invasion complex by using partially noncomplementary peptide nucleic acids (PNAs). Owing to mismatches between 2 PNAs used for invasion, the undesired PNA/PNA duplex, which inhibits invasion complex formation, was destabilized. This approach overcame an inherent challenge in PNA invasion, in particular, undesired PNA/PNA duplex formation resulting from PNA complementarity, thereby enhancing overall invasion efficiency.

SUBMITTER: Shibata M 

PROVIDER: S-EPMC11640769 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature