The Impact of Resident Adipose Tissue Macrophages on Adipocyte Homeostasis and Dedifferentiation.

ABSTRACT: Obesity is concurrent with immunological dysregulation, resulting in chronic low-grade inflammation and cellular dysfunction. In pancreatic islets, this loss of function has been correlated with mature β-cells dedifferentiating into a precursor-like state through constant exposure to inflammatory stressors. As mature adipocytes likewise have the capability to dedifferentiate in vitro and in vivo, we wanted to analyze this cellular change in relation to adipose tissue (AT) inflammation and adipose tissue macrophage (ATM) activity. Using our organotypic AT explant culture method combined with a double-reporter mouse model for labeling ATMs and mature adipocytes, we were able to visualize and quantify dedifferentiated fat (DFAT) cells in AT explants. Preliminary testing showed increased dedif

SUBMITTER: Neugebauer J 

PROVIDER: S-EPMC11640804 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature