Architecture and functional regulation of a plant PSII-LHCII megacomplex.

ABSTRACT: Photosystem II (PSII) splits water in oxygenic photosynthesis on Earth. The structure and function of the C₄S₄M₂-type PSII-LHCII (light-harvesting complex II) megacomplexes from the wild-type and PsbR-deletion mutant plants are studied through electron microscopy (EM), structural mass spectrometry, and ultrafast fluorescence spectroscopy [time-resolved fluorescence (TRF)]. The cryo-EM structure of a type I C₄S₄M₂ megacomplex demonstrates that the three domains of PsbR bind to the stromal side of D1, D2, and CP43; associate with the single transmembrane helix of the redox active Cyt b₅₅₉; and stabilize the luminal extrinsic PsbP, respectively. This megacomplex, with PsbR and PsbY centered around the narrow i

SUBMITTER: Shan J 

PROVIDER: S-EPMC11640958 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature