The Effect of CBM1 and Linker on the Oxidase, Peroxidase and Monooxygenase Activities of AA9 LPMOs: Insight into Their Correlation with the Nature of Reductants and Crystallinity of Celluloses.

ABSTRACT: This study explores the effect of carbohydrate-binding module 1 (CBM1) and the linker on the function of auxiliary activity 9 (AA9) lytic polysaccharide monooxygenases (LPMOs), with a particular focus on monooxygenase activity, using different crystallinity celluloses and electron donors. The tested C1/C4-oxidizing AA9 LPMOs exhibited higher oxidase and peroxidase activities compared to those of the C4-oxidizing AA9 LPMOs. While the presence of CBM1 promoted cellulose-binding affinity, it reduced the oxidase activity of modular AA9 LPMOs. The effect of CBM1 on peroxidase activity was variable and enzyme-specific. Its influence on monooxygenase activity was linked to the type of reductants and the crystallinity of celluloses. Overall, CBM1 improved the monooxygenase activity on high-, mediu

SUBMITTER: Zhao X 

PROVIDER: S-EPMC11641334 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature