Establishment and Validation of an Efficient <i>Agrobacterium Tumefaciens</i>-Mediated Transient Transformation System for <i>Salix Psammophila</i>.

ABSTRACT: Salix psammophila, C. Wang & Chang Y. Yang, a desert-adapted shrub, is recognized for its exceptional drought tolerance and plays a vital role in ecosystem maintenance. However, research on S. psammophila has been limited due to the lack of an efficient and reliable genetic transformation method, including gene functional studies. The Agrobacterium-mediated transient overexpression assay is a rapid and powerful tool for analyzing gene function in plant vivo. In this study, tissue culture seedlings of S. psammophila were utilized as the recipient materials, and the plant expression vector pCAMBIA1301, containing the GUS reporter gene, was transferred into the seedlings via an Agrobacterium-mediated method. To enhance the efficiency of the system, th

SUBMITTER: Yang Y 

PROVIDER: S-EPMC11641538 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature