Image Analysis Using the Fluorescence Imaging of Nuclear Staining (FINS) Algorithm.

ABSTRACT: Finding appropriate image analysis techniques for a particular purpose can be difficult. In the context of the analysis of immunocytochemistry images, where the key information lies in the number of nuclei containing co-localised fluorescent signals from a marker of interest, researchers often opt to use manual counting techniques because of the paucity of available tools. Here, we present the development and validation of the Fluorescence Imaging of Nuclear Staining (FINS) algorithm for the quantification of fluorescent signals from immunocytochemically stained cells. The FINS algorithm is based on a variational segmentation of the nuclear stain channel and an iterative thresholding procedure to count co-localised fluorescent signals from nuclear proteins in other channels. We present exp

SUBMITTER: Bramwell LR 

PROVIDER: S-EPMC11641597 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature