In Vitro and Computational Response of Differential Catalysis by Phlebia brevispora BAFC 633 Laccase in Interaction with 2,4-D and Chlorpyrifos.

ABSTRACT: Enzymes secreted by white rot fungi (WRF), such as laccase, offer a promising approach for the treatment of hazardous xenobiotic compounds. This study conducted a comprehensive analysis of the impact of the pesticides 2,4-dichlorophenoxyacetic acid (2,4-D) and chlorpyrifos on the laccase of Phlebia brevispora BAFC 633 through in vitro and bioinformatics analyses. The fungal strain was shown to be tolerant to both pesticides, with notable morphological and ultrastructural alterations in the mycelium. Laccase activity and two isoenzymes (53 and 70 kDa) were detected in all initial treatments. The laccase was concentrated for subsequent catalytic evaluation in the presence of both pesticides, showing high stability at a pH of 3.6 and a temperature range of 50-60 °C. The lacI gen

SUBMITTER: Ayala Schimpf AR 

PROVIDER: S-EPMC11641778 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature