Development of Primer Panels for Amplicon Sequencing of Human Parainfluenza Viruses Type 1 and 2.

ABSTRACT: Human parainfluenza viruses (hPIVs) are major contributors to respiratory tract infections in young children worldwide. Despite their global significance, genomic surveillance of hPIV1 and hPIV2 had not previously been conducted in Russia. This study aimed to develop a robust amplicon-based sequencing protocol for these viruses. The designed primer sets were tested on clinical samples containing hPIV RNA to evaluate their performance and efficiency. Sequencing results demonstrated high-quality genome data and efficient amplification across various Ct values. As a result, 41 hPIV1 and 13 hPIV2 near-complete genome sequences were successfully obtained from clinical specimens collected in Saint Petersburg (Russia). Phylogenetic analysis of the HN gene sequences showed that Russian hPIV1 strai

SUBMITTER: Mansour O 

PROVIDER: S-EPMC11641795 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature