Development of a Multiplex RT-qPCR Method for the Identification and Lineage Typing of Porcine Reproductive and Respiratory Syndrome Virus.

ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV) is the pathogen that causes porcine reproductive and respiratory syndrome (PRRS), leading to abortion of sows and the manifestation of respiratory diseases in piglets. PRRSV strains are categorized into two distinct genotypes: PRRSV-1 and PRRSV-2. PRRSV-2 can be further classified into several lineages, including sub-lineage 1.8 (NADC30-like), sub-lineage 1.5 (NADC34-like), lineage 8 (HP-PRRSV-like), lineage 5 (VR-2332-like), and lineage 3 (QYYZ-like), all of which are prevalent in China. In order to identify PRRSV-1 and PRRSV-2, two primer-probe combinations were designed, targeting the M gene. In order to further differentiate the five lineages of PRRSV-2, another five primer-probe combinations were designed, targeting the Nsp2

SUBMITTER: Tao C 

PROVIDER: S-EPMC11642648 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature