A mathematical model clarifies the ABC Score formula used in enhancer-gene prediction.

ABSTRACT: Enhancers are discrete DNA elements that regulate the expression of eukaryotic genes. They are important not only for their regulatory function, but also as loci that are frequently associated with disease traits. Despite their significance, our conceptual understanding of how enhancers work remains limited. CRISPR-interference methods have recently provided the means to systematically screen for enhancers in cell culture, from which a formula for predicting whether an enhancer regulates a gene, the Activity-by-Contact (ABC) Score, has emerged and has been widely adopted. While useful as a binary classifier, it is less effective at predicting the quantitative effect of an enhancer on gene expression. It is also unclear how the algebraic form of the ABC Score arises from the underlying mole

SUBMITTER: Nasser J 

PROVIDER: S-EPMC11642778 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature