Effect of Meropenem on Conjugative Plasmid Transfer in <i>Klebsiella pneumoniae</i>.

ABSTRACT: Plasmid-mediated resistance is a major mechanism that contributes to the gradual decline in the effectiveness of antibiotics from different classes, including carbapenems. Antibiotics can significantly contribute to the efficiency of plasmid transfer between bacterial strains. To investigate the potential effect of an antibiotic on the efficacy of conjugative plasmid transfer, we conducted mating experiments with Klebsiella pneumoniae strains. Donor strains of K. pneumoniae that carry plasmids with bla_KPC or bla_OXA-48 carbapenemase genes and recipient plasmid-free K. pneumoniae strains were used in matings. Matings were conducted on the agar with or without meropenem at 1/8×, 1/4×, or 1/2×MIC against the respective recipients. In the

SUBMITTER: Kondratieva DA 

PROVIDER: S-EPMC11642842 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature