Distinct differentiation trajectories leave lasting impacts on gene regulation and function of V2a interneurons.

ABSTRACT: During development, early regionalization segregates lineages and directs diverse cell fates. Sometimes, however, distinct progenitors produce analogous cell types. For example, V2a neurons, are excitatory interneurons that emerge from different anteroposterior progenitors. V2a neurons demonstrate remarkable plasticity after spinal cord injury and improve motor function, showing potential for cell therapy. To examine how lineage origins shape their properties, we differentiated V2a neurons from hPSC-derived progenitors with distinct anteroposterior identities. Single-nucleus multiomic analysis revealed lineage-specific transcription factor motifs and numerous differentially expressed genes related to axon growth and calcium handling. Bypassing lineage patterning via transcription factor-in

SUBMITTER: Elder NH 

PROVIDER: S-EPMC11642877 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature