Control of Epithelial Tissue Organization by mRNA Localization.

ABSTRACT: mRNA localization to specific subcellular regions is common in mammalian cells but poorly understood in terms of its physiological roles^1-6,7. This study demonstrates the functional importance of Net1 mRNA, which we find prominently localized at the dermal-epidermal junction (DEJ) in stratified squamous epithelia. Net1 mRNA accumulates at DEJ protrusion-like structures that interact with the basement membrane and connect to a mechanosensitive network of microfibrils. Disrupting Net1 mRNA localization in mouse epithelium alters DEJ morphology and keratinocyte-matrix connections, affecting tissue homeostasis. mRNA localization dictates Net1 protein distribution and its function as a RhoA GTPase exchange factor (GEF). Altered RhoA activity is in turn sufficient

SUBMITTER: Mason DE 

PROVIDER: S-EPMC11643025 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature