Matrix porosity is associated with <i>Staphylococcus aureus</i> biofilm survival during prosthetic joint infection.

ABSTRACT: Biofilms are a cause of chronic, non-healing infections. Staphylococcus aureus is a proficient biofilm forming pathogen commonly isolated from prosthetic joint infections that develop following primary arthroplasty. Extracellular adhesion protein (Eap), previously characterized in planktonic or non-biofilm populations as being an adhesin and immune evasion factor, was recently identified in the exoproteome of S. aureus biofilms. This work demonstrates that Eap and its two functionally orphaned homologs EapH1 and EapH2, contribute to biofilm structure and prevent macrophage invasion and phagocytosis into these communities. Biofilms unable to express Eap proteins demonstrated increased porosity and reduced biomass. We describe a role for Eap proteins in vivo using a mous

SUBMITTER: Bhattacharya M 

PROVIDER: S-EPMC11643045 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature