Glucose-Sensing ChREBP Protein in the Pathogenesis of Dia-betic Retinopathy.

ABSTRACT: Glucose-sensing ChREBP and MondoA are transcriptional factors involved in lipogenic, inflammatory, and insulin signaling pathways implicated in metabolic disorders; however, limited ocular studies have been conducted on these proteins. We aimed to investigate the potential role of ChREBP in pathogenesis of diabetic retinopathy (DR). We used diabetic human and mouse retinal cryosections analyzed by immunohistochemistry. qRT-PCR was performed to quantify gene expression. To explore the role of ChREBP in rods, we generated caChREBP^RP mice with constitutively active (ca) ChREBP. These mice underwent retinal function testing, followed by proteomic analysis using LC-MS. Furthermore, ARPE-19 cells were infected with lentiviral particles expressing human ChREBP (ARPE-19^ChREBP

SUBMITTER: Starr CR 

PROVIDER: S-EPMC11643094 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature