Impact of a <i>Withania somnifera</i> and <i>Bacopa monnieri</i> Formulation on SH-SY5Y Human Neuroblastoma Cells Metabolism Through NMR Metabolomic.

ABSTRACT: Objectives: This study investigates the effectiveness of an herbal formulation, STRESSLESS (ST-65), which combines ashwagandha (Withania somnifera) and bacopa (Bacopa monnieri), on SH-SY5Y human neuroblastoma cells. Given the rising interest in natural compounds for neuroprotection and stress alleviation, we aimed to explore the cellular and molecular effects of this formulation. Methods: Utilizing a nuclear magnetic resonance (NMR) metabolomic approach and ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS), we identified key bioactive compounds in ST-65, including withanolides from ashwagandha and bacosides from bacopa. Results: Our findings indicate that ST-65 treatment significantly alters the metabolic profile

SUBMITTER: D'Elia M 

PROVIDER: S-EPMC11643393 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature