Effective Decolorization of Poly-γ-Glutamic Acid Fermentation Broth by Integrated Activated Carbon Adsorption and Isoelectric Point Precipitation of Glutamic Acid.

ABSTRACT: Poly-γ-glutamic acid (γ-PGA) is widely used in the field of biomedicine, food, agriculture, and ecological remediation. For the biosynthesis of γ-PGA, the pigments and remaining glutamate are two big problems that impede γ-PGA production by fermentation, and a trade-off between the decolorization rate and γ-PGA recovery rate during the purification process was found. The optimized static activated carbon adsorption conditions for treating the 2-times diluted cell-free supernatant (i.e., feed solution) was as follows: 0.51% 200-mesh, 1000 iodine value, coal-based activated carbon, pH 6.0, 140 min, and 40 °C. Under the optimized conditions, the decolorization rate reached 94.42%, and the recovery rate of γ-PGA was 94.22%. During the adsorption process, the pigments were adsorbed on the activ

SUBMITTER: Si X 

PROVIDER: S-EPMC11643718 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature