An Environmentally Compatible and Less Costly (Greener) Microwave Digestion Method of Bone Samples Using Dilute Nitric Acid for Analysis by ICP-MS.

ABSTRACT: An environmentally compatible and less costly (greener) analytical method for the digestion of bone meal samples using microwave-assisted dilute nitric acid (HNO₃) was developed and optimized. The method, employing a mixture of 1 mL concentrated HNO₃ and 4 mL of deionized water, offered a comparable performance to the conventional method using 5 mL of concentrated HNO₃. The accuracy of the method was validated by using certified reference material NIST 1486 (Bone Meal); percentage recoveries were within ±15% for all eight certified elements. Statistical analysis revealed no significant differences (p > 0.05) in percentage recoveries between the green and conventional methods for all elements except calcium. The greenness of the developed method was e

SUBMITTER: Bussan DD 

PROVIDER: S-EPMC11643719 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature