Toward Standardization of a Lung New Approach Model for Toxicity Testing of Nanomaterials.

ABSTRACT: This study represents an attempt toward the standardization of pulmonary NAMs and the development of a novel approach for toxicity testing of nanomaterials. Laboratory comparisons are challenging yet essential for identifying existing limitations and proposing potential solutions. Lung cells cultivated and exposed at the air-liquid interface (ALI) more accurately represent the physiology of human lungs and pulmonary exposure scenarios than submerged cell and exposure models. A triculture cell model system was used, consisting of human A549 lung epithelial cells and differentiated THP-1 macrophages on the apical side, with EA.hy926 endothelial cells on the basolateral side. The cells were exposed to silver nanoparticles NM-300K for 24 h. The model used here showed to be applicable for asses

SUBMITTER: Elje E 

PROVIDER: S-EPMC11643904 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature