Effects of Different Concentrations of AmB on the Unsaturated Phospholipid-Cholesterol Membrane Using the Langmuir Monolayer and Liposome Models.

ABSTRACT: Amphotericin B (AmB) causes toxicity to the erythrocyte membrane, leading to hemolysis, which limits the clinically effective dose for AmB intravenous therapy in invasive fungal infections. The molecular mechanism by which AmB adheres to the membrane of erythrocytes is the key factor in causing AmB to be toxic to the membrane of erythrocytes, but it is not yet fully understood; the mechanism by which AmB adheres to the liquid microdomains with higher fluidity formed by cholesterol and unsaturated phospholipids remains especially unclear. This study examined the adsorption of AmB at different concentrations, 5, 45, 85, and 125 μg/mL, on unsaturated phospholipid membranes containing 50 mol% cholesterol. The thermodynamic properties and structure of DOPC monolayers and DOPC/cholesterol mixed

SUBMITTER: Wang J 

PROVIDER: S-EPMC11644019 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature