Gene expression profile of cervical tissue compared to exfoliated cells: impact on biomarker discovery.

ABSTRACT:

Background

Exfoliated cervical cells are used in cytology-based cancer screening and may also be a source for molecular biomarkers indicative of neoplastic changes in the underlying tissue. However, because of keratinization and terminal differentiation it is not clear that these cells have an mRNA profile representative of cervical tissue, and that the profile can distinguish the lesions targeted for early detection.

Results

We used whole genome microarrays (25,353 unique genes) to compare the transcription profiles from seven samples of normal exfoliated cells and one cervical tissue. We detected 10,158 genes in exfoliated cells, 14,544 in the tissue and 7320 genes in both samples. For both sample types the genes grouped into the same major gene ontology (GO) categories in

SUBMITTER: Steinau M 

PROVIDER: S-EPMC1164412 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature