Development and evaluation of a Gal4-mediated LUC/GFP/GUS enhancer trap system in Arabidopsis.

ABSTRACT:

Background

Gal4 enhancer trap systems driving expression of LacZ and GFP reporters have been characterized and widely used in Drosophila. However, a Gal4 enhancer trap system in Arabidopsis has not been described in the primary literature. In Drosophila, the reporters possess a Gal4 upstream activation sequence (UAS) as five repeats (5XUAS) and lines that express Gal4 from tissue specific enhancers have also been used for the ectopic expression of any transgene (driven by a 5XUAS). While Gal4 transactivation has been demonstrated in Arabidopsis, wide use of a trap has not emerged in part because of the lack of detailed analysis, which is the purpose of the present study.

Results

A key feature of this study is the use of luciferase (LUC) as the primary reporter and rsGFP-GUS

SUBMITTER: Engineer CB 

PROVIDER: S-EPMC1164422 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature