Detection of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> in Australian Cattle and Sheep by Analysing Volatile Organic Compounds in Faeces.

ABSTRACT: Paratuberculosis is a debilitating disease of ruminants that causes significant economic loss in both cattle and sheep. Early detection of the disease is crucial to controlling the disease; however, current diagnostic tests lack sensitivity. This study evaluated the potential for volatile organic compounds (VOCs) detected by gas chromatography and an electronic nose (eNose) for use as diagnostic tools to differentiate between Map-infected and non-infected cattle and sheep. Solid-phase micro-extraction gas chromatography-mass spectrometry (SPME GC-MS) was used to quantify VOCs from the headspace of faecal samples (cattle and sheep), and partial least squares-discriminant analysis (PLS-DA) was used to determine the suitability as a diagnostic tool. Both the cattle and sheep models had high s

SUBMITTER: Hodgeman R 

PROVIDER: S-EPMC11644260 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature