Enhancing the Stability and Anticancer Activity of <i>Escherichia coli</i> Asparaginase Through Nanoparticle Immobilization: A Biotechnological Perspective on Nano Chitosan.

ABSTRACT: There is a shortage in the experimental research directly comparing the effectiveness of different nanoparticles in boosting asparaginase (ASNase) activity. This study assessed the impact of various nanoparticles on enhancing ASNase activity, stability, and anticancer effects through immobilization. Escherichia coli ASNase was immobilized on different nanoparticles, and its efficiency was measured. The research included analyzing the enzyme's secondary structure, stability, activity at different temperatures, kinetic parameters, shelf life, and activity in blood serum. The anticancer efficacy was determined by measuring the IC₅₀. The study also investigated the anticancer mechanisms by examining the enzyme's toxicity on cancer cells, focusing on apoptosis indicators like

SUBMITTER: Alharthi F 

PROVIDER: S-EPMC11644295 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature