Characteristics of Callus and Cell Suspension Cultures of Highbush Blueberry (<i>Vaccinium corymbosum</i> L.) Cultivated in the Presence of Different Concentrations of 2,4-D and BAP in a Nutrient Medium.

ABSTRACT: In this work, cultures of callus and suspension cells originating from leaves of sterile highbush blueberry (Vaccinium corymbosum L.) plants were obtained and characterized. For their active growth and production of phenolic compounds, a combination of 2,4-D at a concentration of 0.34-2.25 µM and BAP at a concentration of 0.45-2.25 µM is effective. An increase in the phytohormone concentration leads to a slowdown in culture formation and reduces their ability to synthesize phenolic compounds. When cultivating V. corymbosum suspension cells over a year (12 passages), they not only retain the ability to synthesize phenolic compounds but also enhance it. By the 12th passage, the content of TSPC in suspension cells reaches 150 mg/g DW, the content of flavonoids reaches 100 mg/g D

SUBMITTER: Rybin DA 

PROVIDER: S-EPMC11644369 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature