Monitoring Biochemical Changes of Neuroblastoma Cells in Early Stages After X-Ray Exposure by Using Fourier-Transform Infrared Spectroscopy.

ABSTRACT: X-ray radiation treatments are largely adopted in radiotherapy, and Fourier-transform infrared microspectroscopy (μ-FTIR) has already been demonstrated to be a useful instrument for monitoring radiotherapy effects. Previous works in this field have focused on studying the changes occurring in cells when they are fixed immediately after the irradiation or 24 and 48 h later. In the present paper, changes occurring in SH-SY5Y neuroblastoma cells in the first hours after the irradiation are examined to obtain information on the processes taking place in this not-yet-investigated time window by using μ-FTIR. For this purpose, cell samples were fixed immediately after X-ray exposure, and 2 and 4 h after irradiation and investigated along with unexposed cells. Different data analysis procedures w

SUBMITTER: Esposito R 

PROVIDER: S-EPMC11644395 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature