Comparing Results from 2-D and 3-D Phenotyping Systems for Soybean Root System Architecture: A 'Comparison of Apples and Oranges'?

ABSTRACT: Typically, root system architecture (RSA) is not visible, and realistically, high-throughput methods for RSA trait phenotyping should capture key features of developing root systems in solid substrates in 3D. In a published 2-D study using thin rhizoboxes, vermiculite as a growing medium, and photography for imaging, triplicates of 137 soybean cultivars were phenotyped for their RSA. In the transition to 3-D work using X-ray computed tomography (CT) scanning and mineral soil, two research questions are addressed: (1) how different is the soybean RSA characterization between the two phenotyping systems; and (2) is a direct comparison of the results reliable? Prior to a full-scale study in 3D, we grew, in pots filled with sand, triplicates of the Casino and OAC Woodstock cultivars that had s

SUBMITTER: Belzile F 

PROVIDER: S-EPMC11644466 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature