Highly Efficient Agrobacterium tumefaciens Mediated Transformation of Oil Palm Using an EPSPS-Glyphosate Selection System.

ABSTRACT: Oil palm (Elaeis guineensis Jacq.) is the most efficient oil-producing crop globally, yet progress in its research has been hampered by the lack of effective genetic transformation systems. The EPSPS gene, encoding 5-enolpyruvylshikimate-3-phosphate synthase, has been used as a transgenic selection marker in various crops, including rice and soybean. This study evaluated the EPSPS/glyphosate selection system for oil palm transformation. We constructed a binary expression vector, pCGlyDESCLI-C, containing the TIPS-EiEPSPS selection marker from goosegrass and the mScarlet-I red fluorescent reporter gene. This vector was introduced into oil palm embryonic callus (EC) via Agrobacterium-mediated transformation. After optimizing the transformation steps,

SUBMITTER: Ouyang C 

PROVIDER: S-EPMC11644814 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature