Full-Length Transcriptomes Reconstruction Reveals Intraspecific Diversity in Hairy Vetch (<i>Vicia villosa</i> Roth) and Smooth Vetch (<i>V. villosa</i> Roth var. <i>glabrescens</i>).

ABSTRACT: Hairy vetch (Vicia villosa Roth) and smooth vetch (V. villosa Roth var. glabrescens) are important cover crops and legume forage with great economic and ecological values. Due to the large and highly heterozygous genome, full-length transcriptome reconstruction is a cost-effective route to mining their genetic resources. In this study, a hybrid sequencing approach combining SMRT and NGS technologies was applied. The results showed that 28,747 and 40,600 high-quality non-redundant transcripts with an average length of 1808 bp and 1768 bp were generated from hairy vetch and smooth vetch, including 24,864 and 35,035 open reading frames (ORFs), respectively. More than 96% of transcripts were annotated to the public databases, and around 25% of isoforms underwent alternativ

SUBMITTER: Kong W 

PROVIDER: S-EPMC11644882 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature